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Related Concept Videos

Cell Specific Gene Expression01:58

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Updated: May 3, 2026

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma
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In vitro expression of genes encoding HIF1α, VEGFA, PGE2 synthases, and PGE2 receptors in feline oral squamous cell

Walaa Hamed Shaker Nasry1, Juan Carlos Rodriguez-Lecompte1, Chelsea K Martin1

  • 1Department of Pathology and Microbiology, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, Prince Edward Island, Canada.

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
|February 11, 2025
PubMed
Summary
This summary is machine-generated.

Feline oral squamous cell carcinoma (FOSCC) cells express genes involved in prostaglandin E2 (PGE2) pathways and angiogenesis. SCCF2 cells show inducible gene expression and respond to PGE2 and EP4 antagonism, indicating EP4

Keywords:
CD147EP1EP2EP3EP4HIF1αPGE2PTGES1PTGES2PTGES3RT-qPCRVEGFAangiogenesisfeline oral squamous cell carcinomainflammation

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Area of Science:

  • Oncology
  • Molecular Biology
  • Veterinary Medicine

Background:

  • Feline oral squamous cell carcinoma (FOSCC) is an aggressive cancer with poor prognosis.
  • Prostaglandin E2 (PGE2)-mediated inflammation and angiogenesis are implicated in human oral cancer, but their role in FOSCC is unknown.
  • Understanding these pathways is crucial for developing targeted therapies for FOSCC.

Purpose of the Study:

  • To investigate the expression of key genes in PGE2 synthesis, signaling, and angiogenesis in FOSCC cell lines in vitro.
  • To explore the regulatory effects of serum and exogenous PGE2 on these genes.
  • To assess the response of FOSCC cells to PGE2 and EP4 receptor antagonism.

Main Methods:

  • Reverse-transcription quantitative real-time PCR (RT-qPCR) was used to analyze gene expression in FOSCC cell lines (SCCF1-3).
  • Cells were treated with serum to assess inducibility of target genes.
  • Exogenous PGE2 and an EP4 antagonist (L-161,982) were used to study cellular responses.

Main Results:

  • Genes encoding PGE2 synthases (PTGES1-3), PGE2 receptors (EP1-4), HIF1A, and VEGFA were expressed in FOSCC cells.
  • Serum induced expression of PTGES1, PTGES3, EP4, and VEGFA in SCCF2 cells, and VEGFA in SCCF1 cells.
  • PGE2 treatment stimulated HIF1A and CD147 expression in SCCF2 cells, and modulated COX2 and EP4 expression.
  • SCCF3 cells exhibited high HIF1A but low VEGFA expression compared to other cell lines.
  • SCCF2 cells responded to PGE2 and EP4 antagonism, with L-161,982 affecting COX2 and EP4 expression.

Conclusions:

  • FOSCC cells express genes critical for PGE2 production, signaling, and angiogenesis.
  • The EP4 receptor pathway is active in FOSCC and may be a therapeutic target.
  • Further research into EP4 activity in FOSCC is warranted to explore its potential as a treatment target.