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Related Experiment Video

Updated: Jul 10, 2026

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

Published on: March 15, 2012

Protoplasts from Aspergillus nidulans.

W J van den Broek, H G Stunnenberg, L M Wennekes

Microbios

|January 1, 1979

Summary

This study details an effective enzyme system for producing large quantities of Aspergillus nidulans protoplasts. Maximal lytic activity and protoplast yield were achieved using specific enzyme combinations and optimized conditions.

Area of Science:

Mycology
Enzymology
Cell Biology

Background:

Filamentous fungi like Aspergillus nidulans are crucial in biotechnology but require efficient protoplast production for genetic manipulation.
Existing methods for Aspergillus protoplast isolation can be inefficient, limiting large-scale applications.

Purpose of the Study:

To develop and optimize a highly effective lytic enzyme system for massive micro/macro-scale protoplast production from Aspergillus nidulans.
To investigate factors influencing protoplast yield and stability.

Main Methods:

Utilized a lytic enzyme mixture containing chitinase and alpha-(1 leads to 3)-glucanase activities.
Optimized pre-incubation with 2-deoxy-D-glucose, fungal age, culture conditions, pH, and osmotic stabilizer concentration.

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Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

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Last Updated: Jul 10, 2026

Customization of Aspergillus niger Morphology Through Addition of Talc Micro Particles

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Published on: March 15, 2012

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software

Published on: July 24, 2021

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

Incubated Aspergillus nidulans mycelium under specific conditions (pH 6.5, 0.3-0.4 M (NH4)2SO4, 30°C) for protoplast release.

Main Results:

Maximal lytic activity correlated with the presence of both chitinase and alpha-(1 leads to 3)-glucanase.
Protoplast release was significantly enhanced by 2-deoxy-D-glucose pre-incubation.
Produced 2.5 x 10^8 stable protoplasts from 40 mg of mycelium within 3 hours under optimized conditions.
Observed aberrant hyphal regeneration from vacuolated protoplasts at low osmotic stabilizer concentrations.

Conclusions:

The described lytic enzyme system is highly effective for large-scale Aspergillus nidulans protoplast production.
Optimized conditions, including specific enzyme activities and pre-treatments, are critical for maximizing protoplast yield and stability.
Further research into the observed peculiar regeneration phenomenon is warranted.