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Updated: May 28, 2025
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
Published on: June 8, 2019
Synchronous Dual-Cycle Amplification System Based on Programmable Ago-DNA Polymerase for Detecting Tumor-Related
Qiuyuan Lin1, Yicheng Huang2, Junchao Chen1
1Department of Laboratory Medicine, Key Laboratory of Clinical Laboratory Technology for Precision Medicine, School of Medical Technology and Engineering, Fujian Medical University, Fuzhou 350005, China.
None:
Detecting nucleic acid biomarkers is essential for early cancer diagnosis and monitoring. Traditional methods (e.g., exponential isothermal amplification (EXPAR)) adopt DNA polymerases and endonucleases for nucleic acid amplification and analysis, but they are limited by the dependence on specific sequences and nonspecific amplification. Here, we establish a programmable and universal nucleic acid diagnostic platform named Functional Junction Molecule Unveil (FJMU) for microRNAs detection. FJMU incorporates Argonaute (Ago) nuclease and DNA polymerase to accomplish nucleic acid detection in a single reaction. It utilized a specially designed repetitive sequence template to achieve synchronized dual-cycling amplification of biomarkers. The FJMU method eliminates the reliance on restriction endonucleases such as Nt.BstNBI used in EXPAR. FJMU showed high specificity and stability in detecting colorectal cancer-related microRNAs. A comparison of the detection results between the FJMU method and the reverse transcription-polymerase chain reaction (RT-PCR) for clinical serum samples suggests that the FJMU method is potentially feasible for clinical applications. FJMU provided a programmable platform for detecting microRNAs and further explored the Ago-mediated nucleic acid detection technology.
