A light-driven molecular motor-polypeptide conjugate supports controlled cell uptake.

Area of Science:

• Biotechnology
• Materials Science
• Cell Biology

Background:

• Light-driven molecular motors (MMs) offer precise control over cellular functions.
• Challenges include poor biocompatibility and solubility, limiting their biological applications.

Purpose of the Study:

• To develop a biocompatible and soluble molecular motor (MM) system for enhanced cellular response.
• To investigate the light-induced behavior and cellular interactions of the novel P-MM conjugate.

Main Methods:

• Synthesized a polypeptide-conjugated MM (P-MM) using click chemistry, linking a propargyl-derivatized MM to a poly-L-glutamic acid carrier (P).
• Evaluated P-MM stability, rotational capability, cell uptake, subcellular targeting, cytotoxicity, and reactive oxygen species production under 405 nm irradiation.

Main Results:

• P-MM retained MM stability and rotational function upon 405 nm light exposure.
• Light-induced conformational changes significantly enhanced P-MM cellular uptake compared to controls.
• P-MM demonstrated minimal cytotoxicity and reactive oxygen species generation, indicating good biocompatibility.

Conclusions:

• The P-MM conjugate successfully integrates molecular motor functionality with a biocompatible, targeted nanocarrier.
• Light irradiation enhances cellular responses, showcasing potential for controlled biological applications.
• This approach paves the way for advanced light-responsive therapeutic and diagnostic strategies.