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Amino Acid Metabolism-Related Gene Kynureninase (KYNU) as a Prognostic Predictor and Regulator of Diffuse Large

Yu Zhang1, Shi Feng2, Liemei Lv3

  • 1Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250021, Shandong, China.

Biochemical Genetics
|February 11, 2025
PubMed
Summary

Amino acid metabolism reprogramming impacts diffuse large B-cell lymphoma (DLBCL) prognosis. Kynureninase (KYNU) is a potential biomarker, with its inhibition reducing DLBCL cell proliferation and inducing apoptosis.

Keywords:
Amino acid metabolismDLBCLImmune therapyPrognosisTumor microenvironment

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Area of Science:

  • Biochemistry
  • Oncology
  • Genetics

Background:

  • Amino acid metabolism dysregulation influences cancer development and the tumor microenvironment.
  • The specific role of amino acid metabolism-related genes in diffuse large B-cell lymphoma (DLBCL) is not well understood.

Purpose of the Study:

  • To investigate the impact of amino acid metabolism-related genes on DLBCL using bioinformatics.
  • To identify prognostic biomarkers and therapeutic targets for DLBCL.

Main Methods:

  • Consensus clustering to identify prognostic subgroups.
  • LASSO-Cox regression to develop a predictive risk model.
  • RT-qPCR, cell proliferation assays, and flow cytometry to validate findings.

Main Results:

  • Amino acid metabolism reprogramming shows prognostic value in DLBCL.
  • A risk model identified kynureninase (KYNU) as a potential prognostic biomarker.
  • KYNU was upregulated in DLBCL cells, and its inhibition reduced proliferation and induced apoptosis.

Conclusions:

  • Amino acid metabolism significantly impacts DLBCL.
  • KYNU may serve as a valuable prognostic biomarker and therapeutic target for DLBCL.
  • Findings support improved DLBCL prognosis assessment and potential new therapeutic strategies.