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Updated: May 28, 2025

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
Published on: August 16, 2012
Holographic tomographic volumetric additive manufacturing.
Maria Isabel Álvarez-Castaño1, Andreas Gejl Madsen2, Jorge Madrid-Wolff3,4
1Laboratory of Applied Photonics Devices, School of Engineering, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. maria.alvarezcastano@epfl.ch.
Holographic phase modulation significantly enhances volumetric 3D printing efficiency and resolution. This advanced technique enables rapid fabrication of complex microscale objects, even in scattering materials like cell-laden hydrogels.
Area of Science:
- Additive Manufacturing
- Optical Engineering
- Bioprinting
Background:
- Photopolymerization-based 3D printing technologies are advancing rapidly.
- Tomographic Volumetric Additive Manufacturing (TVAM) enables rapid, support-free microfabrication.
- Previous TVAM utilized incoherent light patterning.
Purpose of the Study:
- To investigate the potential of holographic phase modulation for volumetric 3D printing.
- To improve light projection efficiency and precision in TVAM.
- To demonstrate fabrication of complex microstructures in various materials.
Main Methods:
- Implemented a light engine with holographic phase modulation for TVAM.
- Utilized computer-generated holograms with tiled holograms and point-spread-function shaping.
- Tested fabrication in acrylates and scattering, cell-laden hydrogels.
Main Results:
- Achieved over a 20-fold improvement in light projection efficiency compared to amplitude coding.
- Enabled diffraction-limited resolution and precise light control.
- Successfully fabricated millimetric 3D objects with 31 μm negative features in under a minute.
- Demonstrated printing in soft cell-laden hydrogels at high cell densities.
Conclusions:
- Holographic phase modulation offers a significant advancement for volumetric 3D printing.
- This technology enables high-efficiency, high-resolution, and rapid fabrication of microscale objects.
- The method is suitable for printing in both standard resins and complex biological materials.
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