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Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
Published on: December 14, 2017
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Rpl12 is a conserved ribophagy receptor.
Yuting Chen1,2, Jiaxin Hu3, Pengwei Zhao1
1Department of Biochemistry and Department of Hepatobiliary and Pancreatic Surgery of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Nature Cell Biology
|February 11, 2025
Summary
Rpl12 is identified as a conserved receptor for ribophagy, a process crucial for ribosome turnover. This finding is vital for cellular homeostasis, impacting starvation, aging, and disease models.
Area of Science:
- Cell Biology
- Molecular Biology
- Autophagy Research
Background:
- Ribophagy is a selective autophagic process regulating ribosome turnover.
- NUFIP1, a mammalian ribophagy receptor, lacks homologues in yeast and nematodes.
Purpose of the Study:
- To identify conserved ribophagy receptors beyond NUFIP1.
- To elucidate the role of Rpl12 in ribophagy and cellular homeostasis across different organisms.
Main Methods:
- Investigated Rpl12-Atg8s binding interactions.
- Analyzed Atg1-mediated Rpl12 phosphorylation and its role in Atg11 association.
- Utilized Caenorhabditis elegans and Drosophila melanogaster models to study ribophagy deficiency.
Main Results:
- Rpl12 identified as a conserved ribophagy receptor.
- Disruption of Rpl12-Atg8s binding causes ribosomal protein and rRNA accumulation.
- Rpl12 phosphorylation enhances Atg11 binding, triggering ribophagy during starvation.
- Ribophagy deficiency accelerates cell death, impairs development, shortens lifespan, and causes motor deficits.
- RPL12 overexpression rescues starvation, aging, and Aβ-induced movement defects in flies.
Conclusions:
- Rpl12 acts as a conserved ribophagy receptor essential for ribosome metabolism.
- Ribophagy mediated by Rpl12 is critical for cellular homeostasis, organismal health, and longevity.
- Rpl12 is a potential therapeutic target for age-related and neurodegenerative disorders.
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