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Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study
Rosemeire Arai Yoshida1, Tiago Bertola Lobato2, Renata Gorjão2
1Postgraduate Program in Dentistry, Department of Individuals with Special Needs, Cruzeiro do Sul University, São Paulo, Brazil.
Background:
Elevated levels of inflammatory mediators in saliva have been described in individuals with cerebral palsy (CP).
Objective:
The aim of this study was to detect and quantify the pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Prevotella intermedia in the saliva of adolescents with CP.
Methods:
This is a cross-sectional study with adolescents with CP to detect periodontopathogens from saliva samples. Initially, saliva was collected from the CP (n = 34) and control groups (n = 31), followed by the gingival index (GI) for distribution of the groups of individuals with CP and control with gingivitis (bleeding on probing ≥ 10%) and without gingivitis. Bacterial DNA was extracted from saliva samples for detection of periodontopathogens by quantitative PCR (q-PCR). Data were analyzed by Mann-Whitney and Kruskal-Wallis tests, analysis of variance t-test (ANOVA) with Tukey-Kramer post-hoc tests (p < 0.05).
Results:
The quantification of DNA of periodontopathogens in saliva samples showed that adolescents with CP present a variability (22.93-39.56) in the detection of P. gingivalis and that some subjects with CP and gingivitis (n = 4) present high quantification of P. gingivalis (ranging 39.56-37.65), although no significant difference was found between the groups (p > 0.05). A significant contrast was observed for the pathogen P. intermedia when comparing the difference in the control group (p = 0.0396). No major differences were detected in the quantification of periodontopathogens evaluated between the control group and CP.
Conclusion:
Adolescents with CP showed variability in the detection of DNA of periodontopathogens, especially a great variation in the detection of P gingivalis in saliva of CP with gingivitis.
