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Related Concept Videos

GPCR Desensitization01:12

GPCR Desensitization

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G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
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A 12-Step Desensitization Protocol for Calaspargase Pegol-mknl.

Minh H N Nguyen1, Amanda Memken2, Panida Sriaroon1

  • 1Division of Allergy and Immunology (MN, PS, MH), Department of Pediatrics, University of South Florida Morsani College of Medicine, Tampa, FL.

The Journal of Pediatric Pharmacology and Therapeutics : JPPT : the Official Journal of PPAG
|February 12, 2025
PubMed
Summary

Hypersensitivity reactions (HSRs) to asparaginase treatments for acute lymphoblastic leukemia (ALL) are common. A novel desensitization protocol for calaspargase pegol-mknl successfully managed HSRs in a pediatric ALL patient.

Keywords:
asparaginasecalaspargasedesensitization protocolhypersensitivityleukemia

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Area of Science:

  • Oncology
  • Immunology
  • Pharmacology

Background:

  • Asparaginase is a cornerstone therapy for childhood acute lymphoblastic leukemia (ALL).
  • Hypersensitivity reactions (HSRs) to bacterial asparaginase limit its use, even with PEGylated formulations like pegaspargase.
  • Recombinant asparaginase Erwinia chrysanthemi is an alternative, but HSRs can still occur.

Purpose of the Study:

  • To report a case of a pediatric patient with relapsed ALL experiencing HSRs to both pegaspargase and recombinant Erwinia asparaginase.
  • To describe a novel desensitization protocol for calaspargase pegol-mknl in a patient with multiple prior HSRs.
  • To evaluate the safety and efficacy of this desensitization protocol, assessing for adverse events and serum asparaginase activity.

Main Methods:

  • Case report of an 11-year-old boy with relapsed ALL.
  • Administration of a novel desensitization protocol for calaspargase pegol-mknl.
  • Monitoring for hypersensitivity reactions and assessment of serum asparaginase activity.

Main Results:

  • The patient experienced HSRs to both pegaspargase and recombinant Erwinia asparaginase.
  • The novel desensitization protocol for calaspargase pegol-mknl was administered without any adverse events.
  • Adequate serum asparaginase activity was maintained throughout the treatment.

Conclusions:

  • This case demonstrates the successful use of a novel desensitization protocol for calaspargase pegol-mknl in a pediatric patient with acute lymphoblastic leukemia who had prior hypersensitivity reactions.
  • The protocol was safe and effective, allowing for continued treatment with adequate therapeutic drug levels.