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Updated: May 28, 2025

A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
Published on: August 31, 2014
Identification of a Novel HIV-1 Recombinant Form Comprising CRF01_AE and Subtype C in Hebei Province, China
Yapeng Guan1, Jun Wang2, Xinli Lu3
1Department of Food and Drug Engineering, Shijiazhuang College of Applied Technology, Shijiazhuang, Hebei, China.
Background:
Circulating recombinant form (CRF) 01_AE and subtype C are two HIV-1 subtypes. In recent years, novel HIV-1 recombinant forms have become more and more prevalent in China; however, new HIV-1 CRF01_AE/C recombinant forms are less prevalent nationwide.
Objective:
Our study aimed to investigate new recombinant forms between different HIV-1 subtypes and evaluate their transmission risk among men who have sex with men (MSM) in Hebei, China.
Methods:
The near full-length genome (NFLG) of HIV-1 was identified using the analyses of the phylogenetic tree and gene breakpoints.
Results:
In the present work, we have reported a novel HIV-1 recombinant form composed of CRF01_AE and subtype C. The NFLG of this CRF01_AE/C form contained eight gene subregions, with four subtype C gene segments inserted into the CRF01_AE backbone, consisting of I CRF01_AE (790-1,171 nt), Ⅱ subtype C (1,172-1,840 nt), Ⅲ CRF01_AE (1,841-5,089 nt), Ⅳ subtype C (5,090-5,666 nt), Ⅴ CRF01_AE (5,667-6,317 nt), Ⅵ subtype C (6,318-8,586 nt), Ⅶ CRF01_AE (8,587-9,246 nt), and Ⅷ subtype C (9,247-9,409 nt). This new recombinant form was identified as CRF140_0107.
Conclusion:
The study suggested that it is important to monitor HIV-1 diversity to reduce HIV- 1 transmission in China.
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