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Harnessing haploid-inducer mediated genome editing for accelerated maize variety development
Lina Li1,2, Xiao Fu1, Xiantao Qi1
1State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Plant Biotechnology Journal
|February 12, 2025
Summary
Haploid induction and genome editing (HI-Edit/IMGE) efficiently generates targeted mutations in maize, accelerating crop breeding. This study validates HI-Edit/IMGE for industrial application, demonstrating high induction and editing rates in commercial varieties.
Area of Science:
- Plant Science
- Genetics
- Agricultural Biotechnology
Background:
- Haploid induction and genome editing (HI-Edit/IMGE) offers a powerful approach for crop improvement.
- Technical aspects and suitability for the maize seed industry require thorough evaluation.
Purpose of the Study:
- To develop and assess HI-Edit/IMGE maize lines for targeted mutation generation.
- To evaluate the efficiency and accuracy of HI-Edit/IMGE in commercial maize backgrounds.
Main Methods:
- Engineered three HI-Edit/IMGE maize lines (EditWx, EditSh, EditWx&Sh) using CRISPR-Cas9 targeting Waxy1 (Wx1) and Shrunken2 (Sh2) genes.
- Utilized the haploid inducer CHOI3 and characterized copy numbers and mutant alleles (mtl, dmp) facilitating haploid induction.
- Assessed performance in B73 and six other commercial parental lines.
Main Results:
- Maternal haploid induction efficiencies ranged from 8.55% to 20.89%.
- Targeted mutation rates were between 0.38% and 1.46%.
- EditWx&Sh successfully combined multiple CRISPR-Cas9 systems for simultaneous mutations.
Conclusions:
- HI-Edit/IMGE is industrially applicable for generating targeted mutations via pollination.
- Meticulous Cas9 copy number characterization is crucial for optimizing HI-Edit/IMGE.
- The technology accelerates the integration of edited traits into commercial maize varieties.
