Lymph flow, ionic and biochemical indicators of lymph and blood during hypoxia

In this study, the biochemical parameters and physico-chemical reactions of the body in experimental hypoxia, using a Sprague Dawley Rat Model. Hypoxia changed the dynamics and biochemical parameters of blood and lymph, as well as urine. During hypoxia, there was a change in the osmotic resistance of erythrocytes. Hypoxic training was conducted in a hypoxic animal chamber for 15 days and 30 days for 40 minutes every day. Physical and chemical parameters of blood, lymph and its morphological composition were studied on a hematological analyser, oxygen tension and pH of blood and lymph on an OPTI CCA-TS2 Blood Gas and Electrolyte Analyser. The value of osmotic pressure in the lymph changed slightly from 280.22 ± 2.07 to 293.3±3.1 and 285.6 ± 2.8 mOsm/l, respectively, 15 and 30 days of hypoxia. Urine osmotic pressure decreased by 15.1-10.4%, respectively, compared to the control group. After 15 and 30 days of hypoxia, ion exchange in the blood plasma showed a decrease in the concentration of K+, Cl- ions and an increase in the concentration of Na+ ions in the blood plasma and lymph. Ca2+ concentrations decreased in blood plasma and increased in lymph and urine. The analysis of the osmotic resistance of erythrocytes showed its decrease. Lipid peroxidation of erythrocyte membranes showed a significant increase in the level of malondialdehyde and diene conjugates by 52.2% and 69.6%, as well as a decrease in the activity of superoxide dismutase and catalase by 32% and 29.7%. Hypoxia leads to a decrease in erythrocyte resistance and lipid peroxidation in experimental animals. Shifts in pH on the side of acidosis and disturbances in physico-chemical properties in the blood and lymph were detected. As a result of developing hypoxia in the body, structural and functional rearrangements occur in the whole blood of experimental animals.