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Open-Channel Droplet Microfluidic Platform for Passive Generation of Human Sperm Microdroplets.

Tristan M Nicholson1, Jodie C Tokihiro2, Wan-Chen Tu2

  • 1Department of Urology, School of Medicine, University of Washington, Seattle, Washington 98105, United States.

ACS Biomaterials Science & Engineering
|February 12, 2025
PubMed
Summary

Vitrification offers superior sperm preservation in small volumes. A new microfluidic device passively generates these volumes, maintaining sperm quality for fertility applications.

Keywords:
Microfluidicscryopreservationhuman spermsub-microlitervitrification

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Area of Science:

  • Reproductive Biology
  • Biotechnology
  • Microfluidics

Background:

  • Sperm cryopreservation is crucial for assisted reproduction.
  • Vitrification is a promising technique for preserving sperm quality.
  • Handling small sample volumes for vitrification presents a challenge in fertility labs.

Purpose of the Study:

  • To develop a microfluidic device for generating small-volume human sperm samples.
  • To assess the compatibility of the device with human sperm for cryopreservation.
  • To facilitate the adoption of sperm vitrification in fertility laboratories.

Main Methods:

  • Utilized open-channel microfluidics to create an open droplet generator.
  • Passively generated sub-microliter to microliter volumes of purified human sperm.
  • Evaluated sperm kinematics after processing with the device.

Main Results:

  • The microfluidic device successfully generated small volumes of human sperm samples.
  • The generated samples maintained sperm kinematics, indicating preserved quality.
  • The platform demonstrated compatibility with human sperm.

Conclusions:

  • The developed open droplet generator is effective for preparing small sperm volumes.
  • This microfluidic platform supports sperm quality preservation for vitrification.
  • The technology provides a foundation for implementing advanced sperm cryopreservation techniques in clinical settings.