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Modeling Neuropathic Corneal Pain: Pulled Nerve Approach With Elevated Krt16 Gene Expression
Mohd Afzal Khan1, Gehan Fatima1, Acquah Emmanuel1
1Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, South Korea.
Investigative Ophthalmology & Visual Science
|February 12, 2025
Summary
A novel rodent model for neuropathic corneal pain (NCP) was developed by gently pulling the long ciliary nerve. This model accurately reflects human NCP, aiding in understanding and developing new therapies.
Area of Science:
- Ophthalmology
- Neuroscience
- Pain Research
Background:
- Neuropathic corneal pain (NCP) affects millions globally, yet current animal models are limited.
- Existing models often involve complex surgeries and high risks, hindering research progress.
Purpose of the Study:
- To develop a novel, less invasive rodent model for neuropathic corneal pain (NCP).
- To investigate both structural and functional mechanisms underlying NCP.
- To provide a comprehensive approach for NCP research.
Main Methods:
- A novel 'pulled nerve' model was created by gently uplifting the long ciliary nerve (LCN) in rodents.
- Pain perception and chemical sensitivity were quantified using Stevens' Power Law.
- Compared outcomes with normal control, sham control, and full transection groups.
Main Results:
- The pulled nerve model demonstrated heightened pain perception and altered chemical sensitivity.
- Distinct Alpha values in pain-perception curves differentiated control, pulled nerve, and transection groups.
- Histological analysis showed LCN elongation, corneal changes, and reduced satellite glial cells; Krt16 gene expression was upregulated.
Conclusions:
- The developed rodent model effectively mimics human NCP conditions.
- This model aids in delineating the pathological landscape of NCP.
- It promises to accelerate the development of targeted NCP therapies.
