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Related Experiment Videos

Differences between CSF and plasma Na+ and K+ activities and concentrations.

D C Johnson, L Orlowitz, B M Hitzig

    The American Journal of Physiology
    |May 1, 1985
    PubMed
    Summary

    Ion-selective electrodes (ISE) and flame photometry yield different sodium and potassium measurements in canine plasma and cerebrospinal fluid (CSF). ISE provides accurate activity ratios crucial for thermodynamic equilibrium equations.

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    Area of Science:

    • Neuroscience
    • Clinical Chemistry
    • Analytical Chemistry

    Background:

    • Accurate determination of electrolytes like sodium ([Na+]) and potassium ([K+]) is vital in physiological research.
    • Ion-selective electrodes (ISE) and flame photometry are common methods for electrolyte analysis, but their results can differ.
    • Understanding these differences is crucial for interpreting physiological data, particularly in cerebrospinal fluid (CSF).

    Purpose of the Study:

    • To compare sodium and potassium concentrations determined by ISE and flame photometry in canine plasma and CSF.
    • To evaluate the impact of anesthesia on plasma electrolyte levels.
    • To assess the suitability of different ratio types (activity, molar, molal) for thermodynamic and electroneutrality equations.

    Main Methods:

    Related Experiment Videos

  • Direct potentiometric determination using ion-selective electrodes (ISE).
  • Flame photometry for electrolyte analysis.
  • Analysis of plasma and cisternal cerebrospinal fluid (CSF) from pentobarbital-anesthetized dogs.
  • Electrolyte measurements in solutions with and without albumin and bicarbonate.

    • Main Results:

    • Plasma [Na+] showed no change post-anesthesia, while plasma [K+] decreased by 5%.
    • Sodium activity in CSF and plasma was identical, suggesting no contribution to potential difference.
    • CSF K+ activity was 74% of plasma K+ activity.
    • Activity ratios (ISE) differed from molar and molal ratios for CSF-to-plasma.
    • Flame photometry values differed from ISE values across all sample types.
    • Albumin and bicarbonate affected ISE and flame photometry readings differently.

    Conclusions:

    • ISE provides accurate activity ratios essential for thermodynamic equilibrium equations.
    • Molar concentrations are appropriate for electroneutrality equations and strong ion difference calculations.
    • Methodological choices in electrolyte determination significantly impact physiological interpretations.