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Updated: Jun 17, 2026

ELIME Enzyme Linked Immuno Magnetic Electrochemical Method for Mycotoxin Detection
Published on: October 23, 2009
Coordinative interaction-enhanced aggregation-induced electrochemiluminescence signal enables ultrasensitive
Ya Gao1, Hao Liu1, Shaowei Li1
1Ministry of Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, Collaborative Innovation Center for Advanced Organic Chemical Materials Co-constructed by the Province and Ministry, Hubei Key Laboratory for Precision Synthesis of Small Molecule Pharmaceuticals, College of Chemistry and Chemical Engineering, Hubei University, Wuhan 430062, China.
Abstract:
Aflatoxins B1 (AFB1), as a secondary metabolite of Aspergillus flavus and Aspergillus parasiticus, is the most toxic mycotoxin. It is urgently needed to detect AFB1 pollution in time at ultra-low concentration levels. Aggregation-induced electrochemiluminescence (AIECL) has aroused widespread interest due to the significantly improved ECL response by overcoming aggregation-caused quenching. However, current AIECL probes to satisfy trace AFB1 determination remain a big challenge. Herein, by virtue of hollow UiO-66-NH2 with multiple active sites, 1,1,2,2-tetra(4-carboxylphenyl)ethylene (TCTPE)-functionalized AIECL probe (TH-UiO-66-NH2) was designed and prepared for constructing an aptamer-based sensor toward AFB1 monitoring. Due to the coordination of TCTPE and Zr4+, TCTPE molecules were loaded and largely restricted in hollow UiO-66-NH2, leading to a breakthrough in AIECL signal enhancement. Further combined with magnetic separation and accumulated strand displacement, ultrasensitive and selective detection of AFB1 was achieved with an ultra-low detection limit of 1.04 fg/mL, providing new ideas for food safety supervision.
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