Withanolide glycosides from Physalis volubilis.

Area of Science:

• Natural Product Chemistry
• Pharmacognosy
• Microbiology

Background:

• Physalis volubilis is a plant species with potential medicinal properties.
• Withanolide glycosides are a class of compounds known for diverse biological activities.

Purpose of the Study:

• To isolate and characterize chemical constituents from Physalis volubilis.
• To evaluate the antibacterial potential of isolated compounds.

Main Methods:

• Extraction and isolation of compounds using acetone and methanol.
• Structure elucidation via spectroscopic and spectrometric data analysis and chemical transformations.
• Antibacterial activity screening using qualitative assays and determination of minimum inhibitory concentrations (MICs).

Main Results:

• Four withanolide glycosides (4, 6, 10, 14) and other compounds were isolated.
• Compound 2 exhibited notable antibacterial activity against tested Gram-positive and Gram-negative bacteria.
• MIC values for Compound 2 against Staphylococcus aureus ATCC® 25923™ and MRSA were 62.5 μg/mL and 125 μg/mL, respectively.

Conclusions:

• Physalis volubilis is a source of bioactive withanolide glycosides.
• Compound 2 is a promising candidate for further investigation as an antibacterial agent, especially against resistant strains like MRSA.