Association between GH, PRL, LEP, and PIT-1 gene polymorphisms and growth traits in Indonesian Rambon indigenous
Peni Wahyu Prihandini1, Dwi Nur Happy Hariyono2, Aprilianna Putri Zahara Nafsina Luvita Sari3
1Research Center for Animal Husbandry, Research Organization for Agriculture and Food, National Research and Innovation Agency, Cibinong, Bogor, 16911, Indonesia. peni005@brin.go.id.
Tropical Animal Health and Production
|February 12, 2025
Summary
Genetic markers in cattle growth genes, specifically PIT-1 and LEP, are linked to growth traits like body length and weight. Heterozygous animals showed superior growth, suggesting potential for marker-assisted selection in Rambon cattle.
Area of Science:
- Animal Genetics
- Molecular Biology
- Livestock Science
Background:
- Understanding genetic mechanisms of cattle growth is crucial for livestock improvement.
- Identifying genetic markers associated with growth traits can aid in selective breeding programs.
Purpose of the Study:
- To investigate genetic variations in growth hormone (GH), prolactin (PRL), leptin (LEP), and pituitary-specific transcription factor (PIT-1) genes in Rambon cattle.
- To identify single nucleotide polymorphisms (SNPs) associated with growth characteristics in this indigenous cattle population.
Main Methods:
- Direct sequencing was used to detect 20 SNPs in 31 Rambon cattle.
- Allele and genotype frequencies were analyzed.
- Association analysis was performed between SNPs and growth traits (body length, body weight, chest girth, shoulder height).
Main Results:
- A non-synonymous mutation (g.10976A>C) was identified in the PIT-1 gene.
- Significant associations were found between LEP SNP g.12238G>A and body length.
- PIT-1 SNPs (g.10976A>C and g.11398A>C) were associated with body weight, chest girth, and shoulder height.
- Heterozygous individuals for associated SNPs exhibited enhanced growth performance.
Conclusions:
- Specific polymorphisms in LEP and PIT-1 genes are associated with key growth traits in Rambon cattle.
- These identified SNPs could serve as potential markers for marker-assisted selection to improve growth performance.
- No significant associations were found for SNPs in GH and PRL genes with the studied growth traits.
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