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Isolated White Lupin Proteins Beneficially Modulate the Intestinal Microbiota Composition in Rats.

Luis A Rubio¹, Giulia Chiesa²

¹Department of Animal Nutrition and Sustainable Production, Estación Experimental del Zaidin (CSIC), Profesor Albareda 1, 18008 Granada, Spain.

|February 13, 2025

Summary

This summary is machine-generated.

White lupin protein isolate (LPI) beneficially modulates rat gut microbiota. Milk proteins (lactalbumin and casein) also altered microbiota, with lactalbumin showing a healthier profile than casein.

Keywords:

Lupinus albus casein intestinal microbiota composition lactalbumin protein isolates rat

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Area of Science:

Microbiology
Nutrition Science
Gastroenterology

Background:

Legume-based diets are known to positively influence gut microbiota.
These beneficial effects are partly attributed to the protein components of legumes.

Purpose of the Study:

To investigate the impact of different protein sources on fecal microbiota composition in rats.
To compare the effects of milk proteins (lactalbumin and casein) with white lupin protein isolate (LPI).

Main Methods:

Rats were fed diets containing lactalbumin (LA), casein (CAS), or LPI for 28 days.
Fecal microbiota composition and functionality were analyzed using qPCR and Illumina sequencing.
Statistical analyses included ANOSIM, Discriminant Analysis, LEfSe, and PCA.

Main Results:

Significant differences in microbiota composition and functionality were observed among the three dietary groups.
The LPI diet led to lower levels of Enterobacteria and Escherichia/Shigella compared to casein and lactalbumin diets, respectively.
The casein diet resulted in lower Bifidobacteria and Lactobacilli compared to the other diets.

Conclusions:

White lupin protein isolate (LPI) likely beneficially modulates rat intestinal microbiota.
A lactalbumin-based diet promoted a healthier microbiota composition compared to casein.
Casein diet also modulated microbiota, potentially improving bowel movement frequency and lipid metabolism.