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Anti-Methicillin-Resistant Staphylococcus aureus Efficacy of Layer-by-Layer Silver Nanoparticle/Polyacrylic
Julinthip Puttawong1, Mingkwan Yingkajorn2, Pasarat Khongkow1,3
1Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110, Thailand.
Abstract:
The emergence of methicillin-resistant Staphylococcus aureus (MRSA) is still posing a global challenge in healthcare settings. This bacterial strain is a cause of severe periprosthetic infection, thereby impairing the success of implant insertion. To address this issue, implant surface modification is required. Herein, we developed a novel multilayered silver nanoparticle/polyacrylic acid-coated Ti plate (AgNPs/PAA/Ti) using an in-house dip coater. AgNPs were synthesized and characterized. The dip-coating process was optimized based on the dipping rate, evaporation time, and coating cycle number. Uniform and reproducible coatings were achieved on Ti surfaces, with consistency verified through SEM analysis. The average size of the AgNPs was approximately 36.50 ± 0.80 nm with a PDI of 0.443 ± 0.025, and the zeta potential was measured at around -23.3 ± 2.0 mV. The maximum coating thickness of 83.5 ± 1.3 µm was observed at 15 cycles of dip coating. Moreover, our developed AgNPs/PAA/Ti plate showed both antimicrobial and biofilm-resistant performance, while also exhibiting enhanced biocompatibility with cultured MG63 osteosarcoma cells, maintaining cell viability greater than 70%. We envisage that this material holds significant promise as a candidate for medical implant devices, offering protection against MRSA-associated infection at insertion sites with low vascularity in the future.
Insights
A new silver nanoparticle/polyacrylic acid coating on titanium plates effectively combats methicillin-resistant Staphylococcus aureus (MRSA) infections. This innovative surface modification enhances implant safety and biocompatibility for medical devices.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Infectious Disease Research
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) poses a significant threat in healthcare, causing severe periprosthetic infections that compromise implant success.
- Effective strategies are needed to prevent MRSA colonization and infection on medical implants.
Purpose of the Study:
- To develop and characterize a novel multilayered silver nanoparticle/polyacrylic acid-coated titanium (AgNPs/PAA/Ti) plate.
- To evaluate the antimicrobial, antibiofilm, and biocompatibility properties of the developed AgNPs/PAA/Ti coating.
Main Methods:
- Synthesis and characterization of silver nanoparticles (AgNPs).
- Optimization of a dip-coating process for uniform AgNPs/PAA coatings on titanium surfaces.
- Assessment of coating morphology using SEM, particle size, PDI, zeta potential, and thickness.
- Evaluation of antimicrobial and antibiofilm activity against MRSA.
- In vitro biocompatibility testing with MG63 osteosarcoma cells.
Main Results:
- Uniform and reproducible AgNPs/PAA coatings were successfully fabricated on Ti surfaces.
- Characterization confirmed AgNP size (~36.5 nm), PDI (0.443), zeta potential (-23.3 mV), and maximum coating thickness (83.5 µm at 15 cycles).
- The AgNPs/PAA/Ti plate demonstrated significant antimicrobial and biofilm-resistant properties.
- Enhanced biocompatibility was observed, with MG63 cell viability maintained above 70%.
Conclusions:
- The developed AgNPs/PAA/Ti coating offers a promising solution for preventing MRSA infections associated with medical implants.
- This material exhibits excellent antimicrobial efficacy, biofilm resistance, and biocompatibility.
- The novel coating holds potential for application in medical devices, particularly at sites prone to infection.
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