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Quercetin and Silybin Decrease Intracellular Replication of Piscirickettsia salmonis in SHK-1 Cell.

Mick Parra¹, Katherin Izquierdo², Meraiot Rubio¹

¹Laboratory of Natural Products Chemistry and Their Applications, Centre of Aquatic Biotechnology, Faculty of Chemistry and Biology, University of Santiago of Chile, Santiago 9170022, Chile.

International Journal of Molecular Sciences

|February 13, 2025

Summary

This summary is machine-generated.

Quercetin and silybin show potential as antivirulence therapies against Piscirickettsia salmonis, reducing pathogen replication in salmon cells. Further research is needed to optimize their application in aquaculture.

Keywords:

Piscirickettsia salmonis SHK-1 flavonids intracellular replication quercetin silybin

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Area of Science:

Aquatic animal health
Bacteriology
Pharmacology

Background:

Piscirickettsia salmonis poses a significant threat to the Chilean salmon industry, necessitating novel control strategies beyond antibiotics.
Antivirulence therapy offers a promising alternative by targeting pathogen virulence without inhibiting growth.
Polyphenolic compounds are being investigated for their potential antimicrobial and antivirulence properties.

Purpose of the Study:

To evaluate the efficacy of quercetin and silybin in reducing intracellular Piscirickettsia salmonis replication.
To assess the impact of these compounds on salmonid cell lines (SHK-1 and RTgutGC) and phagocytic capacity.

Main Methods:

Three infection protocols were employed using SHK-1 cells: 24-hour co-incubation, pre-incubation, and post-incubation.
Rainbow trout intestinal epithelial cells (RTgutGC) were used to evaluate compound effects in a different cell type.
The influence of quercetin and silybin on the phagocytic capacity of SHK-1 cells was assessed.

Main Results:

Quercetin and silybin significantly decreased intracellular P. salmonis replication in SHK-1 cells during 24-hour co-incubation.
These compounds did not exhibit the same inhibitory effect on P. salmonis replication in RTgutGC cells.
Both quercetin and silybin reduced the phagocytic capacity of SHK-1 cells upon co-incubation.

Conclusions:

Quercetin and silybin demonstrate potential as antivirulence agents against Piscirickettsia salmonis in specific salmon cell lines.
The observed reduction in phagocytosis warrants further investigation regarding the immune response modulation.
These findings support the development of novel therapeutic strategies for controlling P. salmonis infections in aquaculture.