Related Experiment Video

Updated: May 28, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

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Biological Significance and Therapeutic Promise of Programmed Ribosomal Frameshifting

Miora Bruna Marielle Ramamonjiharisoa1,2, Sen Liu1,2

  • 1Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), Wuhan 430068, China.

International Journal of Molecular Sciences
|February 13, 2025
PubMed

Insights

Programmed Ribosomal Frameshifting (PRF) alters gene expression during translation. Understanding PRF

Area of Science:

  • Molecular Biology
  • Genetics
  • Virology

Background:

  • Programmed Ribosomal Frameshifting (PRF) is a key translational control mechanism.
  • PRF alters the mRNA reading frame, producing out-of-frame proteins essential for cellular functions.
  • Dysregulated PRF is implicated in disease pathogenesis, especially viral infections.

Purpose of the Study:

  • To review the biological significance of PRF in cellular homeostasis and disease.
  • To highlight PRF's role in viral infections and immune responses to SARS-CoV-2 mRNA vaccines.
  • To underscore the therapeutic potential of targeting PRF modulation.

Main Methods:

  • Literature review of PRF mechanisms and functions.
  • Analysis of PRF's role in viral replication and pathogenesis.
  • Examination of PRF's impact on vaccine-induced immune responses.

Main Results:

  • PRF is critical for maintaining cellular homeostasis and viral replication.
  • PRF can trigger immune responses, as observed with SARS-CoV-2 mRNA vaccines.
  • PRF efficiency is modulated by cellular factors, indicating context-dependent regulation.

Conclusions:

  • A comprehensive understanding of PRF is vital for developing novel therapeutic strategies.
  • Targeting PRF offers potential for treating viral infections and improving vaccine efficacy.
  • Further research into PRF modulation is essential for clinical applications.
Keywords:
eIF5Apolyamineprogrammed ribosomal frameshiftingtranslational regulation

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