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Related Experiment Video

Updated: May 28, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

Biological Significance and Therapeutic Promise of Programmed Ribosomal Frameshifting.

Miora Bruna Marielle Ramamonjiharisoa^1,2, Sen Liu^1,2

¹Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), Key Laboratory of Fermentation Engineering (Ministry of Education), Wuhan 430068, China.

International Journal of Molecular Sciences

|February 13, 2025

Summary

Programmed Ribosomal Frameshifting (PRF) alters gene expression during translation. Understanding PRF

Keywords:

eIF5A polyamine programmed ribosomal frameshifting translational regulation

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Published on: August 7, 2021

Area of Science:

Molecular Biology
Genetics
Virology

Background:

Programmed Ribosomal Frameshifting (PRF) is a key translational control mechanism.
PRF alters the mRNA reading frame, producing out-of-frame proteins essential for cellular functions.
Dysregulated PRF is implicated in disease pathogenesis, especially viral infections.

Purpose of the Study:

To review the biological significance of PRF in cellular homeostasis and disease.
To highlight PRF's role in viral infections and immune responses to SARS-CoV-2 mRNA vaccines.
To underscore the therapeutic potential of targeting PRF modulation.

Main Methods:

Literature review of PRF mechanisms and functions.
Analysis of PRF's role in viral replication and pathogenesis.
Examination of PRF's impact on vaccine-induced immune responses.

Main Results:

PRF is critical for maintaining cellular homeostasis and viral replication.
PRF can trigger immune responses, as observed with SARS-CoV-2 mRNA vaccines.
PRF efficiency is modulated by cellular factors, indicating context-dependent regulation.

Conclusions:

A comprehensive understanding of PRF is vital for developing novel therapeutic strategies.
Targeting PRF offers potential for treating viral infections and improving vaccine efficacy.
Further research into PRF modulation is essential for clinical applications.