Genetic Variation Analysis and Development of KASP Marker for Leaf Area and Hight in Southern-Type Populus deltoides

Populus deltoides holds significant ecological and economic importance and is a crucial gene donor for the world's staple poplar varieties. To select and breed P. deltoides with improved agronomic traits, nine growth and leaf traits were examined in 375 different genotypes, assessing their genetic diversity and performing correlation and comprehensive ranking analyses. Phenotyping results were then utilized to screen a total of 2,009,263 SNP (single nucleotide polymorphism) loci significantly associated with the nine phenotypic traits. A total of 45 SNP loci exhibited significant associations with growth traits based on a general linear model (GLM) analysis. By analyzing the Linkage disequilibrium (LD) block of five SNP loci with significant leaf area and height, we identified five candidate genes related to leaf area and height. Three of the five SNP loci were successfully validated using KASP (kompetitive allele-specific PCR) assays. One loci Chr08_16007979 was closely linked with leaf area, and two loci Chr05_12148738, and Chr05_17106547 were closely linked with height. The developed functional KASP markers offer valuable insights for subsequent further marker-assisted breeding and genetic improvement studies in southern-type poplars.