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Related Experiment Video

Updated: May 28, 2025

Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
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A Simple, Cost-Effective Microfluidic Device Using a 3D Cross-Flow T-Junction for Producing Decellularized

Farah Kamar1, Connor J Gillis2, Grace Bischof3

  • 1Department of Medical Biophysics, Western University, London, Ontario, Canada.

Journal of Biomedical Materials Research. Part A
|February 13, 2025
PubMed
Summary
This summary is machine-generated.

Researchers developed a new microfluidic method to create small, uniform microcarriers from decellularized adipose tissue. These extracellular matrix microcarriers effectively support human mesenchymal stromal cell growth in bioreactors, advancing cell therapy manufacturing.

Keywords:
T‐junction cross‐flow deviceadipose‐derived stromal cellsdecellularized adipose tissueextracellular matrix (ECM)microcarriersmicrodroplet formationmicrofluidics

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Therapy Manufacturing

Background:

  • Human mesenchymal stromal cells (MSCs) hold significant therapeutic potential but face manufacturing challenges due to traditional 2D culture limitations.
  • Existing extracellular matrix (ECM)-derived microcarriers, while promising, are often large and non-uniform, hindering optimal MSC expansion and cell delivery.
  • There is a need for scalable, reproducible methods to produce small, uniform microcarriers for advanced MSC applications.

Purpose of the Study:

  • To develop a novel microfluidic approach for producing small, uniform, ECM-derived microcarriers.
  • To evaluate the capacity of these microcarriers to support human adipose-derived stromal cell (hASC) growth and ECM production in vitro.
  • To establish a cost-effective and reusable platform for MSC expansion.

Main Methods:

  • Fabrication of microcarriers using a modified 3D T-junction microfluidic device with decellularized adipose tissue (DAT) as the ECM source.
  • Optimization of flow rates and photo-crosslinking with rose bengal to achieve desired microcarrier size and stability.
  • In vitro culture of hASCs on the developed microcarriers within spinner flask bioreactors for 14 days.

Main Results:

  • Successful generation of small (mean diameter 196 ± 47 μm) and monodisperse microcarriers using the microfluidic device.
  • Demonstrated high production rates and cost-effectiveness of the microfluidic fabrication method.
  • Confirmed robust attachment, proliferation, and ECM production of hASCs on DAT microcarriers over 14 days in bioreactors.

Conclusions:

  • The novel microfluidic device enables efficient production of uniform, cell-supportive ECM microcarriers.
  • These microcarriers represent a promising platform for scalable MSC expansion and potential for minimally invasive cell delivery.
  • The developed method offers a cost-effective and reusable solution for advancing cell therapy manufacturing.