Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scalable differentiation of human cardiac organoids from iPSCs generates cardiac tissues for cardiac cell therapy.

Theranostics·2026
Same author

Allogeneic Immune Cell Perfusion Inhibits the Growth of Vascularized 3D In Vitro Tumor Models, Induces Vascular Regression and Desmoplasia, but Promotes Tumor Cell Invasion.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Plant-Derived Viral Nanoparticles Enable Simultaneous Guidance of Neuronal Cell Outgrowth and Targeting of Neurodifferentiation Pathways.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Mass Production of Uniform Embryoid Bodies by Acoustic Standing Waves.

Small methods·2025
Same author

Modulating Collagen I Expression in Fibroblasts by CRISPR-Cas9 Base Editing of the Collagen 1A1 Promoter.

International journal of molecular sciences·2025
Same author

Porous collagen scaffolds enable endothelial lumen formation in vitro under both static and dynamic growth conditions.

Journal of biomedical materials research. Part B, Applied biomaterials·2024

Related Experiment Video

Updated: May 28, 2025

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
09:35

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Published on: June 16, 2020

9.7K

Engineered In Vitro Multi-Cell Type Ventricle Model Generates Long-Term Pulsatile Flow and Modulates Cardiac Output

Christoph Kuckelkorn1, Ebru Aksoy2, Natalija Stojanovic1

  • 1Department of Dental Materials and Biomaterials Research, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074, Aachen, Germany.

Advanced Healthcare Materials
|February 13, 2025
PubMed
Summary

Researchers engineered a novel in vitro left ventricle model using human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) on a biofunctionalized membrane. This model generates stable pulsatile flow, mimicking cardiac function and responding to drugs.

Keywords:
cardiomyocyteengineered ventriclein vitro modelpulsatile flow

More Related Videos

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening
10:39

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

Published on: April 15, 2017

12.7K
Magnetic Adjustment of Afterload in Engineered Heart Tissues
09:40

Magnetic Adjustment of Afterload in Engineered Heart Tissues

Published on: May 5, 2020

5.8K

Related Experiment Videos

Last Updated: May 28, 2025

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices
09:35

Preclinical Cardiac Electrophysiology Assessment by Dual Voltage and Calcium Optical Mapping of Human Organotypic Cardiac Slices

Published on: June 16, 2020

9.7K
Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening
10:39

Automated Contraction Analysis of Human Engineered Heart Tissue for Cardiac Drug Safety Screening

Published on: April 15, 2017

12.7K
Magnetic Adjustment of Afterload in Engineered Heart Tissues
09:40

Magnetic Adjustment of Afterload in Engineered Heart Tissues

Published on: May 5, 2020

5.8K

Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Stem Cell Technology

Background:

  • In vitro cardiac models are crucial for drug discovery and disease modeling.
  • Existing models often lack the complexity to replicate native heart function, particularly pulsatile flow.
  • Human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) offer a promising cell source for cardiac tissue engineering.

Purpose of the Study:

  • To develop and validate a novel in vitro left ventricle model using biofunctionalized membranes and iPSC-CMs.
  • To assess the model's ability to generate pulsatile flow and mimic physiological conditions.
  • To evaluate the model's utility for drug testing and studying cardiac function.

Main Methods:

  • Engineering a hemispherical polydimethylsiloxane (PDMS) membrane coated with polydopamine and laminin 511 E8 fragments.
  • Culturing human iPSC-CMs, alone or in co-culture with cardiac fibroblasts or endothelial cells, on the membranes.
  • Utilizing a newly developed bioreactor for long-term cell culture (up to 28 days) and flow measurements.
  • Analyzing drug response, cardiac gene expression, and cell morphology.

Main Results:

  • The engineered ventricles demonstrated continuous beating and stable pulsatile flow generation for 28 days.
  • A theoretical cardiac output of up to 4 µL min⁻¹ was achieved, indicating synchronized contraction and stable cell adhesion.
  • iPSC-CMs exhibited physiological responses to cardioactive drugs (carbachol, isoproterenol), with measurable changes in heart rate and cardiac output.

Conclusions:

  • The developed bioengineered ventricle effectively serves as an in vitro left ventricle model.
  • The model supports iPSC-CM culture, differentiation, and long-term functional stability.
  • It accurately mimics physiological flow and demonstrates responsiveness to pharmacological stimuli, validating its use in preclinical studies.