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Updated: Jul 16, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
Published on: May 29, 2014
Methods for Pluripotent Stem Cell Characterization: A Narrative Review
Fadoua Temsamani1, Assia Agalit1, Karima Idrissi Serhrouchni1
1Laboratory of Histology, Embryology and Cytogenetics, Laboratory of Life and Health Sciences Research, Faculty of Medicine and Pharmacy of Tangier, Abdelmalek Essaâdi University, Tangier, MAR.
Abstract:
Pluripotent stem cells (PSCs) have revolutionized medical research by offering significant advancements in regenerative medicine, disease modeling, and drug discovery. Several research and clinical studies have successfully generated PSCs but with varying characterization methodologies. Characterization methods are crucial to guarantee the safety and efficacy of PSCs in research and therapeutic applications. Therefore, there is a need for standardization of characterization methods to ensure their quality, reproducibility, and comparability across laboratories. In this review, the authors discuss the mandatory and non-mandatory assessments used for morphological, molecular, genetic, and functional PSC characterization. We will present their advantages and limits with the aim of providing insights for assessment optimization and helping facilitate the standardization of characterization methods.
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