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A transcriptomic score to classify the inflammation-dysplasia-cancer sequence lesions in inflammatory bowel disease
Anneline Cremer1,2, Nicolas Rosewick2, Maxfield Kelsey3
1Department of Gastroenterology, HUB Erasme University Hospital, Université Libre de Bruxelles, Brussels, Belgium.
A new transcriptomic signature score accurately classifies dysplasia and colorectal cancer in inflammatory bowel disease (IBD) patients. This tool aids in identifying high-risk lesions within the inflammation-dysplasia-cancer sequence.
Area of Science:
- Gastroenterology
- Oncology
- Molecular Biology
Background:
- Inflammatory bowel disease (IBD) increases colorectal cancer risk via the inflammation-dysplasia-cancer (IDC) pathway.
- Early detection of dysplasia and cancer in IBD is crucial for patient management.
Purpose of the Study:
- To develop a transcriptomic signature and score to classify dysplasia and colorectal cancer in IBD patients.
- To identify key genes involved in the IDC sequence.
Main Methods:
- RNA-sequencing data from 134 IBD lesions (normal, inflamed, dysplasia, cancer) were analyzed.
- An ordinal logistic regression identified 27 significant IDC-associated genes.
- A transcriptomic signature score was computed and validated.
Main Results:
- A 27-gene signature, including KCNQ1OT1, correlated with lesion progression.
- The transcriptomic signature score achieved 85.71% accuracy in the exploratory cohort and 90.91% in the validation cohort.
- Unsupervised clustering effectively distinguished between lesion groups.
Conclusions:
- A validated tissue-based transcriptomic score can classify IDC lesions in IBD.
- This score aids in identifying critical genes in IBD-related carcinogenesis.
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