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Mechanism of Astragaloside IV in Promoting Osteogenic Differentiation
Yao Zhu1, Chengyu Lin1, Xudong Zhang1
1Department of Chiropractic, Beijing Jishuitan Hospital, Capital Medical University, 100035, No. 31 Xinjiekou East Street, Xicheng District, Beijing, China.
Background:
This study focuses on exploring the impact of Astragaloside IV [AS-IV] on osteogenic differentiation.
Methods:
Osteogenic differentiation was induced in rat osteoblasts, following which treatment with AS-IV at varied doses was performed. Using Alizarin red staining and alkaline phosphatase (ALP) detection assay, the osteogenic differentiation of the cells was investigated. The expressions of osteogenic differentiation-related genes were determined by quantitative real-time polymerase chain reaction (qRT-PCR). The phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathwayassociated protein expressions were examined using Western blot. After osteoblasts were transfected with protein tyrosine phosphatase non-receptor type 2 (PTPN2) overexpression plasmid, the impact of PTPN2 on osteoblasts treated with AS-IV was examined.
Results:
AS-IV treatment enhanced osteogenic differentiation and up-regulated the expression of osteogenic differentiation-related genes, as well as the levels of p- PI3K/PI3K and p-AKT/AKT, while reducing phosphatase and tensin homolog (PTEN) protein production in osteoblasts. Overexpression of PTEN inhibited osteogenic differentiation, and PTPN2 overexpression counteracted the effects of AS-IV on osteogenic differentiation.
Conclusion:
AS-IV contributing to osteogenic differentiation may be related to the PTPN2-mediated PTEN/PI3K/Akt pathway.
