Taste-Guided Isolation of Bitter Compounds from the Mushroom Amaropostia stiptica Activates a Subset of Human Bitter Taste Receptors
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View abstract on PubMed
Summary
This summary is machine-generated.Researchers identified novel bitter compounds from mushrooms that activate human bitter taste receptors (TAS2Rs). This discovery expands our understanding of bitter taste beyond flowering plants.
Area Of Science
- Mycology
- Human Physiology
- Natural Product Chemistry
Background
- Bitter taste perception is a crucial defense mechanism against toxins.
- Knowledge of bitter substances and their corresponding human bitter taste receptors (TAS2Rs) is heavily biased towards compounds from flowering plants.
- Mushrooms are known to possess bitter compounds, but these remain largely unexplored in relation to TAS2R activation.
Purpose Of The Study
- To identify and characterize novel bitter compounds from mushrooms.
- To investigate the activation of human TAS2Rs by these mushroom-derived compounds.
- To broaden the scope of known bitter agonists beyond flowering plant sources.
Main Methods
- Isolation and structural elucidation of triterpene glucosides (oligoporins D-F) from *Amaropostia stiptica* using spectroscopic analyses.
- Functional screening of isolated oligoporins and the bitter indolalkaloid infractopicrin from *Cortinarius infractus* against all human TAS2Rs.
- Determination of receptor activation profiles and potency, including EC50 values.
Main Results
- Three new triterpene glucosides, oligoporins D-F, were identified from *A. stiptica*.
- All tested mushroom compounds, including oligoporins A-F and infractopicrin, activated at least one TAS2R.
- Oligoporin D demonstrated potent activation of TAS2R46 at submicromolar concentrations, classifying it among the most potent bitter agonists.
Conclusions
- Mushroom-derived compounds, such as oligoporins and infractopicrin, are effective activators of human TAS2Rs.
- The identification of these novel bitter agonists from fungi significantly reduces the knowledge bias in TAS2R research.
- This study highlights the potential of fungi as a source of diverse bitter compounds with implications for taste perception and drug discovery.
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