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Relationship between Genes and microRNAs Involved in the Migration of Cells from the Bone Marrow during Experimental

E I Lebedeva1, A T Shchastniy2, A S Babenka3

  • 1Vitebsk State Order of Peoples' Friendship Medical University, Vitebsk, Republic of Belarus. lebedeva.ya-elenale2013@yandex.ru.

Bulletin of Experimental Biology and Medicine
|February 13, 2025
PubMed

Insights

Bone marrow cell migration contributes to liver fibrosis and cirrhosis. Cxcl12 and Yap1 gene expression correlates with these processes, suggesting potential therapeutic targets for liver disease.

Area of Science:

  • Hepatology and Molecular Biology
  • Immunology and Cell Biology

Background:

  • Liver fibrosis is a complex process involving inflammation and tissue remodeling.
  • Cellular infiltration from bone marrow plays a role in the progression of liver damage.

Purpose of the Study:

  • To investigate the cellular and molecular mechanisms underlying progressive liver fibrosis in a rat model.
  • To identify potential molecular targets for treating liver fibrosis and cirrhosis.

Main Methods:

  • Wistar male rats were subjected to a fibrosis-inducing protocol.
  • Cellular markers (CX3CR1+, CD34+) and gene expression (Cxcl12, Notch1, Notch2, Yap1, miR-3558-3p) were analyzed over time.
  • Correlation analysis was performed to identify relationships between molecular markers and disease progression.

Main Results:

  • Bone marrow-derived CX3CR1+ and CD34+ cells migrated to the liver during fibrosis progression, with CD34+ cell area significantly increasing.
  • Cxcl12 mRNA levels showed dynamic correlations with Notch1, Notch2, and miR-3558-3p throughout fibrosis development.
  • A significant correlation between Cxcl12 and Yap1 mRNA was observed throughout the experiment.

Conclusions:

  • Cell migration from bone marrow, modulated by Cxcl12 and Yap1, is implicated in liver fibrosis and cirrhosis.
  • Cxcl12 and Yap1 represent potential therapeutic targets for mitigating liver fibrosis and cirrhosis progression.
Keywords:
correlation analysisexperimentimmunohistochemistryliver fibrogenesismRNA and microRNA expression

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