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Related Concept Videos

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Related Experiment Video

Updated: May 28, 2025

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Transcription Factor CEBPD-Mediated WTAP Facilitates the Stemness, Growth, Migration and Glycolysis of Glioblastoma

Jiong Geng1, Yun Shao2, Yi Pu2

  • 1Department of Emergency, Nanjing Medical University Affiliated Wuxi People's Hospital, Wuxi, Jiangsu, 214023, China.

Neurochemical Research
|February 13, 2025
PubMed
Summary
This summary is machine-generated.

Wilms tumor 1-associated protein (WTAP) and CCAAT/enhancer-binding protein delta (CEBPD) promote glioblastoma growth. The CEBPD/WTAP axis regulates glioblastoma stem-like cells, offering a potential therapeutic target.

Keywords:
CEBPDGlioblastoma stem like cellsTranscriptionWTAP

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Flow Cytometry-based Drug Screening System for the Identification of Small Molecules That Promote Cellular Differentiation of Glioblastoma Stem Cells
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Area of Science:

  • Oncology
  • Cancer Stem Cell Biology
  • Molecular Oncology

Background:

  • Glioblastoma stem-like cells (GSCs) possess high tumorigenicity and drive glioblastoma (GBM) progression.
  • Wilms tumor 1-associated protein (WTAP) is implicated in GBM malignancy, but its role in GSCs remains undefined.

Purpose of the Study:

  • To investigate the role of WTAP in regulating GSCs function and GBM progression.
  • To elucidate the regulatory relationship between WTAP and CCAAT/enhancer-binding protein delta (CEBPD) in GBM.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and western blot for WTAP and CEBPD expression analysis.
  • In vitro assays: sphere formation, CCK8, EdU, colony formation, flow cytometry, and transwell assays to assess GSCs stemness, proliferation, apoptosis, and migration.
  • Glycolysis evaluation, ChIP assay, dual-luciferase reporter assay, and in vivo tumorigenicity experiments.

Main Results:

  • WTAP and CEBPD expression were elevated in GBM tissues and GSCs.
  • WTAP silencing inhibited GSCs stemness, proliferation, migration, and glycolysis, while promoting apoptosis.
  • CEBPD enhanced WTAP transcription by binding to its promoter; WTAP overexpression counteracted CEBPD knockdown effects.

Conclusions:

  • The CEBPD/WTAP axis is crucial for regulating GSCs function, including stemness, proliferation, migration, and glycolysis.
  • Targeting the CEBPD/WTAP axis presents a promising therapeutic strategy for glioblastoma.