Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

367
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
367
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

5.6K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
5.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparison of Real-time RT-PCR cycle threshold (Ct) values with clinical features and severity of COVID-19 disease among hospitalized patients in the first and second waves of COVID-19 pandemic in Chennai, India.

Journal of clinical virology plus·2023
Same author

Gene expressions and their significance in organoid cultures obtained from breast cancer patient-derived biopsies.

Acta histochemica·2022
Same author

Transcriptional Profiling and Deriving a Seven-Gene Signature That Discriminates Active and Latent Tuberculosis: An Integrative Bioinformatics Approach.

Genes·2022
Same author

Collagen-ZnO Scaffolds for Wound Healing Applications: Role of Dendrimer Functionalization and Nanoparticle Morphology.

ACS applied bio materials·2022
Same author

Dendrimer-Functionalized Metal Oxide Nanoparticle-Mediated Self-Assembled Collagen Scaffold for Skin Regenerative Application: Function of Metal in Metal Oxides.

Applied biochemistry and biotechnology·2021
Same author

Carbon Dots-Mediated Fluorescent Scaffolds: Recent Trends in Image-Guided Tissue Engineering Applications.

International journal of molecular sciences·2021

Related Experiment Video

Updated: May 28, 2025

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
07:12

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Published on: October 26, 2017

7.8K

Fluorescence Enhanced Water-Soluble Ruthenium Complex: Advancing Precision in Cr(VI) Detection and Quantification.

Pooja Rajagopalan1, Sowmiya Saravanan1, Mohan Ranganathan1

  • 1Centre for Analysis, Testing, Evaluation & Reporting Services (CATERS), CSIR-Central Leather Research Institute, Adyar, Chennai, 600 020, India.

Journal of Fluorescence
|February 13, 2025
PubMed
Summary

A new ruthenium-based fluorescence detector offers sensitive and selective detection of toxic chromium (VI) ions. This advancement aids in environmental monitoring and public safety by enabling accurate quantification of chromium (VI) in various samples.

Keywords:
Cr(VI) detection and quantificationCr(VI) estimation in leatherFluorescence sensor for Cr(VI) ionsToxic metal recognition by emission quenchingWater soluble Ru(II) polypyridyl complex

More Related Videos

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
08:57

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

8.4K
Dose Uptake of Platinum- and Ruthenium-based Compound Exposure in Zebrafish by Inductively Coupled Plasma Mass Spectrometry with Broader Applications
08:46

Dose Uptake of Platinum- and Ruthenium-based Compound Exposure in Zebrafish by Inductively Coupled Plasma Mass Spectrometry with Broader Applications

Published on: April 21, 2022

2.3K

Related Experiment Videos

Last Updated: May 28, 2025

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
07:12

Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor

Published on: October 26, 2017

7.8K
A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
08:57

A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting

Published on: March 9, 2017

8.4K
Dose Uptake of Platinum- and Ruthenium-based Compound Exposure in Zebrafish by Inductively Coupled Plasma Mass Spectrometry with Broader Applications
08:46

Dose Uptake of Platinum- and Ruthenium-based Compound Exposure in Zebrafish by Inductively Coupled Plasma Mass Spectrometry with Broader Applications

Published on: April 21, 2022

2.3K

Area of Science:

  • Analytical Chemistry
  • Environmental Science
  • Materials Science

Background:

  • Hexavalent chromium (Cr(VI)) poses significant environmental and health risks due to its high toxicity and carcinogenicity.
  • Accurate and rapid detection of Cr(VI) is crucial for public safety, regulatory compliance, and environmental monitoring.
  • Existing detection methods may lack the sensitivity, selectivity, or speed required for comprehensive Cr(VI) analysis.

Purpose of the Study:

  • To design and develop a novel fluorescence detector for sensitive and selective quantification of chromium (VI) ions.
  • To synthesize and characterize a novel ruthenium(II) complex for use as a fluorescent probe.
  • To evaluate the performance of the developed detector for Cr(VI) detection in aqueous solutions.

Main Methods:

  • Synthesis of a novel ruthenium(II) complex: [Ru(bpy)2(PhDORi)]Cl2, where bpy is 2,2'-bipyridine and PhDORi is (2S,3R)-4-(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)-butane-1,2,3-triol.
  • Characterization of the synthesized complex using analytical and spectroscopic techniques, including UV-Vis absorption and cyclic voltammetry.
  • Investigation of the complex's fluorescence properties and its response to chromate/dichromate anions in aqueous solution.

Main Results:

  • The synthesized ruthenium(II) complex exhibited a metal-to-ligand charge transfer (MLCT) band at approximately 457 nm in aqueous solution.
  • Cyclic voltammetry confirmed reversible one-electron oxidation and reduction processes involving the Ru(II)/Ru(III) couple.
  • The complex displayed intense fluorescence upon excitation at the MLCT band and showed selective emission quenching in the presence of chromate/dichromate ions across a wide concentration range (ppm to ppb).

Conclusions:

  • The novel ruthenium(II) complex serves as an effective fluorophore for the sensitive and selective detection of chromium (VI).
  • The developed fluorescence detector demonstrates a broad linear response and high sensitivity for chromate ion quantification.
  • This work provides a promising tool for the accurate monitoring of toxic chromium (VI) in environmental and public health applications.