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Bimetallic CuRu nanozymes for colorimetric and smartphone-assisted rapid visual hypoxanthine biosensing in serum
Aifang Zhou1, Juan Chen2, Xintong Dong1
1College of Chemistry, Institute of Analytical Chemistry for Life Science, Zhengzhou Key Laboratory of Functional Nanomaterial and Medical Theranostic, Zhengzhou University, Zhengzhou, 450001, China.
Abstract:
Bimetallic Cu0.5Ru0.5 nanozymes were synthesized by alloying Ru with inexpensive Cu through a polyol method for hypoxanthine sensing. The prepared Cu0.5Ru0.5 nanozymes exhibit a peroxidase-like activity. Under the specific enzyme of xanthine oxidase (XOD), hypoxanthine was first converted to xanthine, and then to the final product uric acid, accompanied by the production of H2O2. Cu0.5Ru0.5 nanozymes could catalyze H2O2 to generate •OH and then oxidize colorless TMB to blue oxTMB, resulting in a visible color change. Therefore, the determination of hypoxanthine was achieved by monitoring the absorption value change of oxTMB at 652 nm. The proposed hypoxanthine sensing strategy displayed a high sensitivity and selectivity, with the limit of detection of 0.5 μM. Furthermore, we designed a rapid and convenient platform for hypoxanthine detection using a smartphone with image recognition and data processing function.
