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Updated: May 28, 2025
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A Robotic Platform for High-throughput Protoplast Isolation and Transformation
Published on: September 27, 2016
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Binary division of plastids involves endoplasmic reticulum mediation.
Puja Puspa Ghosh1, Thomas Kadanthottu Kunjumon1, Jaideep Mathur1
1Laboratory of Plant Development and Interactions, Department of Molecular and Cellular Biology, University of Guelph, 50 Stone Road, Guelph, Ontario N1G2W1, Canada.
Journal of Experimental Botany
|February 13, 2025
Summary
The endoplasmic reticulum (ER) plays a crucial role in plastid division. ARC5 protein localization to the ER band constricts the plastid, enabling daughter plastid separation.
Area of Science:
- Cell Biology
- Plant Biology
- Organelle Dynamics
Background:
- Plastids divide via binary fission, a process involving the cytosolic protein ARC5.
- The precise mechanisms of ARC5 recruitment and final plastid separation remain unclear.
Purpose of the Study:
- To investigate the role of the endoplasmic reticulum (ER) in the late stages of plastid division.
- To elucidate the involvement of ER in ARC5-mediated plastid constriction and separation.
Main Methods:
- Time-lapse imaging of Arabidopsis thaliana.
- Utilized stable transgenics expressing fluorescently tagged ER and ARC5 proteins.
Main Results:
- ARC5 protein associates with ER membranes before mid-plastid localization.
- ER band formation around the plastid mid-plane, driven by ARC5-ER association, constricts the plastid.
- Membrane contact sites (MCSs) facilitate tandem plastid-ER dynamics, promoting rotation and separation of daughter plastids.
Conclusions:
- The endoplasmic reticulum is pivotal in facilitating plastid division.
- ER band constriction and ER-plastid dynamics are key mechanisms for plastid separation.
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