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Differential impact of divalent metals on native elongating transcript sequencing (NET-seq) protocols for RNA

Abigail K Huffines1, David A Schneider1

  • 1Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, Birmingham, AL, United States of America.

Plos One
|February 13, 2025
PubMed
Summary

Metal ions like CaCl2 and MnCl2 significantly reduce RNA polymerase I (Pol I) occupancy during native elongating transcript sequencing (NET-seq). However, these metals did not affect RNA polymerase II (Pol II) occupancy, highlighting unique characteristics of different polymerases.

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