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Updated: May 28, 2025

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
Published on: March 30, 2018
Biochemical mechanism regulating antagonistic interactions between aluminum and manganese combine applications in two
Noor Muhammad1, Asifa Hameed2, Imran Haider Shamsi3
1Department of Agronomy, Institute of Crop Science, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, China; Central Cotton Research Institute, Multan Punjab, Pakistan.
Abstract:
About 30-40% of the world land is acidic in nature, aluminum (Al) and manganese (Mn) toxicity co-existed in acidic soils that pose a potential threat in crop production. Antagonistic relationship between Al and Mn was previously described in our study. In the present work, we determined the biochemical mechanism which regulate antagonistic interaction between Al or Mnco-application. The experiment consisted of 9 treatments including co-application of Al and Mn. Overall, we conclude that increasing exogenous Al or Mn concentration negatively affected phenol and flavonoid contents, increased lignin and callose content, disturbed cell ultra-structure in barley genotypes, with sensitive genotype ZU9 being more affected than XZ16. Mn did not influence organic acids secretion and down regulated Mn tolerance genes and organic acid exudation linked genes while Al addition induced the expression of OAs linked genes, OAs secretion were activated and upregulated Mn tolerance genes. Furthermore, co treatments of Al and Mn restored the abnormalities induced by sole Al or Mn application.
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