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Updated: May 2, 2026
Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy
Published on: April 25, 2021
A sheltering ratio fluorescence composite based on BR-CDs for selectivity detection of GSH
Yuqi Wan1, Shuqi Li1, Jiahua Wang2
1State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China.
Abstract:
To distinguish different biothiol species and resolve cysteine interference in fluorescence detection, a fluorescence ratio strategy with shielding effect that specifically responds to GSH without Cys interference was constructed by employing the composite of blue-emitting (B-CDs) and red-emitting carbon dots (R-CDs). The constructed BR-CDs composite showed a decrease in red-emission intensity and simultaneous recovery of blue-emission intensity in the presence of GSH, while the ratio of dual-emission fluorescence intensity, FL440/FL620, showed a superior linear response to GSH in the range of 30-500 μmol/L with a limit of detection (LOD) of 5.27 μmol/L. This designed strategy exhibited excellent performance in the detection of GSH. The BR-CDs complexes were effective in estimating GSH levels in a variety of food matrices, including tomato, pear and potato, with recoveries% ranging from 94.36 % to 112.02 % and RSD% below 0.41 %, demonstrating the potential of the composites for the selective quantitative detection of GSH.
