JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

Prognostic significance of pigmentation and stem cell markers in Indian population of uveal melanoma

  • 0Ocular Pathology, All India Institute of Medical Sciences, New Delhi, India.
The British Journal of Ophthalmology +

|

February 13, 2025

|

February 13, 2025

View abstract on PubMed

Summary

This summary is machine-generated.

This study found that PAX3, CD133, and ABCG2 expression correlates with uveal melanoma (UM) progression and metastasis. However, tumor pigmentation did not correlate with these cancer stem cell markers.

Area Of Science

  • Ophthalmology
  • Oncology
  • Cancer Stem Cell Research

Background

  • Increased pigmentation is linked to cancer stem-cell-like behavior and chemoresistance in uveal melanoma (UM).
  • This study investigates the relationship between pigmentation and cancer stem cell markers in UM patients.

Purpose Of The Study

  • To determine the correlation between pigmentation and cancer stem cell markers in uveal melanoma.
  • To assess the prognostic significance of these markers in UM.

Main Methods

  • Immunohistochemistry and quantitative real-time PCR were used to assess expression of PAX3, CD133, ABCG2, TRYP1, TRYP2, and MITF in 70 UM cases.
  • Kaplan-Meier analysis and Cox-proportional hazards models analyzed correlations with clinicopathological parameters and patient outcomes.

Main Results

  • PAX3, CD133, and ABCG2 proteins were significantly expressed in UM.
  • PAX3, CD133, and ABCG2 expression correlated with high-risk parameters, distant metastasis, and poorer metastasis-free survival.
  • Tumor pigmentation did not correlate with any assessed markers or overall survival.

Conclusions

  • PAX3, MITF, and TRYP2 expression are associated with cancer stem cell markers in UM progression.
  • PAX3, CD133, and ABCG2 are potential biomarkers for UM progression and metastasis.

Keywords:

Related Concept Videos

Pigmentation 01:19

2.3K

The color of the skin is influenced by a number of pigments, including melanin, carotene, and hemoglobin. Recall that melanin is produced by cells called melanocytes, which are found scattered throughout the stratum basale of the epidermis. The melanin is transferred to the keratinocytes via melanosomes.
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...

Skin Cancer 01:30

3.0K

Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...

iPS Cell Differentiation 01:22

2.6K

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.

iPSCs have been successfully used to treat age-related macular degeneration (AMD), a form of...