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Related Experiment Video

Updated: May 28, 2025

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

Published on: April 30, 2016

Advancing plant gene activation with CRISPR-Act3.0.

Ali Movahedi¹, Zhiying Mu², Liming Yang²

¹State Key Laboratory of Tree Genetics and Breeding, College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China; College of Arts and Sciences, Arlington International University, Wilmington, DE 19804, USA.

Trends in Biotechnology

|February 13, 2025

Summary

We enhanced CRISPR-Act3.0 for plant gene activation, improving stability and consistency in complex polyploid genomes. This modified tool offers more reliable genetic engineering solutions for challenging plant research.

Keywords:

CRISPR-Act3.0 CRISPRa Cas12b GOLD-tracrRNA SpRY gR2.0

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Last Updated: May 28, 2025

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Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

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Published on: April 19, 2019

Area of Science:

Plant science
Genetics
Molecular biology

Background:

CRISPR-Act3.0 is a key tool for plant gene activation.
Challenges include structural instability and inconsistent activation in polyploid genomes.

Purpose of the Study:

To strategically modify CRISPR-Act3.0 for enhanced resilience and adaptability.
To improve gene activation precision in complex plant genomes.

Main Methods:

Strategic modifications to the CRISPR-Act3.0 system.
Testing in polyploid plant genomes.

Main Results:

Increased structural stability of the modified CRISPR-Act3.0.
More consistent and reliable gene activation observed.
Improved adaptability in complex plant genomes.

Conclusions:

The modified CRISPR-Act3.0 system shows significant improvements for plant genome engineering.
This enhanced tool addresses limitations of conventional methods in polyploid plants.