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Inconsistent Protein Stability Despite Pre-HECT Domain Helix: Unveiling Variability in HECT Ligases

Çağdaş Dağ1,2, Cansu Deniz Tozkoparan Ceylan1, Cemre Sare Cansız1

  • 1Nanofabrication and Nanocharacterization Center for Scientific and Technological Advanced Research (n2STAR), Koç University, İstanbul, Türkiye.

Protein and Peptide Letters
|February 14, 2025
PubMed
Summary

The N-terminal alpha-helix does not stabilize the HECT domain in HERC5, challenging previous assumptions. This finding impacts the understanding of ubiquitin-like modification systems and HECT ligase structural biology.

Keywords:
Escherichia coli.HECT ligaseHERC5ISGylationbiotechnologyubiquitin

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