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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: May 28, 2025

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Published on: April 21, 2022

Developing flow cytometry for precise evaluation of amphibian sperm viability: technical report.

Leah Jacobs¹, Talisin Hammond¹, Natalie Calatayud²

¹San Diego Zoo Wildlife Alliance, Escondido, CA, USA.

Reproduction, Fertility, and Development

|February 14, 2025

Summary

Flow cytometry offers a reliable method for assessing amphibian sperm viability and concentration. This advanced technique provides a faster, more dependable alternative to traditional microscopy for sperm analysis.

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Last Updated: May 28, 2025

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

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Published on: April 21, 2022

Fluorimetric Techniques for the Assessment of Sperm Membranes

Fluorimetric Techniques for the Assessment of Sperm Membranes

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Two Types of Assays for Detecting Frog Sperm Chemoattraction

Two Types of Assays for Detecting Frog Sperm Chemoattraction

Published on: December 27, 2011

Area of Science:

Veterinary Science
Reproductive Biology
Amphibian Research

Background:

Flow cytometry is increasingly used for cellular analysis in non-domestic animals.
It shows potential for improving sperm quality assessment, concentration counts, and cell sorting efficiency.

Purpose of the Study:

To validate flow cytometry for amphibian sperm analysis.
To compare flow cytometry results with traditional microscopy methods for sperm quality.

Main Methods:

Sperm samples from African clawed frogs (Xenopus laevis) were analyzed.
Both flow cytometry with fluorescent probes and traditional microscopy were employed.

Main Results:

Sperm concentrations showed high correlation between methods, with flow cytometry yielding higher estimates.
Sperm viability measurements were not significantly correlated and differed slightly (~8%) between techniques.

Conclusions:

Flow cytometry slightly overestimates amphibian sperm concentration and viability.
Despite minor discrepancies, flow cytometry is a valuable and reliable tool for amphibian sperm assessment.