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Updated: May 28, 2025

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
An in-depth interfacial study uncovering the effect of emulsifier mixes on small intestinal in vitro lipid digestion
Daphne Michels1, Sarah H E Verkempinck1, Riet Spaepen2
1KU Leuven, Department of Microbial and Molecular Systems (M(2)S), Laboratory of Food Technology and Leuven Food Science and Nutrition Research Centre (LFoRCe), Kasteelpark Arenberg 22, PB 2457, 3001 Leuven, Belgium.
Abstract:
Emulsifiers in blends affect lipolysis properties such as emulsion stability, bile salt and lipase adsorption, and mixed micelle formation, influencing lipolysis kinetics. Understanding how different emulsifiers affect these roles during in vitro digestion is often lacking. This study elucidates mechanisms during emulsifier adsorption, lipolysis, and desorption affecting lipolysis kinetics. Interfacial tension kinetics and rheology of adsorbed layers containing lysolecithin (LL), monoolein (MG), or glyceryl polyethyleneglycol ricinoleate (SYN), or their mixes, were monitored during in vitro lipolysis using the OCTOPUS pendant drop technique. Results were compared with in vitro lipolysis kinetics profiles of emulsions with identical emulsifier compositions. SYN hindered bile salts and lipase adsorption, causing lag phases in lipolysis kinetics, but facilitated removal of lipid digestion products. MG had no significant effect. LL-interfaces showed similarities to the emulsifier blend, indicating its dominance in lipolysis kinetics. These findings provide insights into emulsifier effects on lipolysis processes and in vitro lipolysis kinetics.
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