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High-throughput screening method for glycolipids based on substrate modification and their efficient

Yuanyi Li1, Zhilin Liu1, Yiming Zheng2

  • 1College of Food Science and Light Industry, Nanjing Tech University, Nanjing 211816, China; State Key Laboratory of Materials-Oriented Chemical Engineering, Nanjing Tech University, Nanjing 211816, China.

Bioresource Technology
|February 14, 2025
PubMed
Summary
This summary is machine-generated.

A novel substrate-modified high-throughput screening (HTS) method boosts microbial synthesis. This approach enhances sophorolipids and rhamnolipids production, enabling cost-effective biomanufacturing.

Keywords:
FermentationHigh-yielding strainRhamnolipidsSophorolipids

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Area of Science:

  • Biotechnology
  • Microbial Fermentation
  • Synthetic Biology

Background:

  • High-throughput screening (HTS) is vital for improving microbial synthesis.
  • Genetic editing-based HTS is often unfeasible in non-model microbial strains.
  • Developing alternative HTS methods is crucial for biomanufacturing advancements.

Purpose of the Study:

  • To develop a substrate-modified HTS strategy for non-model microbial strains.
  • To enhance the production of sophorolipids (SLs) and rhamnolipids (RLs).
  • To facilitate cost-effective biomanufacturing through improved microbial yields.

Main Methods:

  • Optimized fermentation medium to increase SLs yield to 248 g/L.
  • Synthesized 2-benzylstearic acid as a substrate for sophorolipids analog production.
  • Employed a dark-phenotype selection and 254 nm absorption for high-yielding SLs strain screening, achieving a 51.1% success rate.
  • Extended the HTS methodology to rhamnolipids (RLs) production.
  • Engineered a microbubble reactor to enhance RLs yield.

Main Results:

  • Achieved a sophorolipids (SLs) yield of 324 g/L with strain A6.
  • Successfully screened high-yielding SLs strains with a 51.1% success rate.
  • Increased rhamnolipids (RLs) yield to 70.3 g/L using the engineered microbubble reactor.

Conclusions:

  • The substrate-modified HTS strategy is effective for non-model strains like Starmerella bombicola.
  • This HTS methodology significantly augments microbial fermentation product yields.
  • The developed approach enables efficient and cost-effective biomanufacturing of valuable compounds.