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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

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Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
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RNA Splicing01:32

RNA Splicing

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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Responses to Heat and Cold Stress02:45

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Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
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Cell Signaling in Plants01:25

Cell Signaling in Plants

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Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
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Transcription01:10

Transcription

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Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
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Alternative Splicing Dynamics in Plant Adaptive Responses to Stress.

Abdulrahman Alhabsi1, Yu Ling2, Martin Crespi3,4

  • 1Laboratory for Genome Engineering and Synthetic Biology, Division of Biological Sciences, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia;

Annual Review of Plant Biology
|February 14, 2025
PubMed
Summary

Plants use alternative splicing (AS) to adapt to environmental stress. Understanding AS mechanisms and leveraging CRISPR-Cas tools can engineer crops for enhanced stress resilience and sustainable agriculture.

Keywords:
RNAabiotic stressalternative splicingalternative splicing editingbiotic stresspre-mRNAtranscriptional controltranscriptome engineering

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Area of Science:

  • Plant molecular biology
  • Gene regulation
  • Genomics

Background:

  • Plants possess complex molecular networks for stress adaptation.
  • Gene regulation, including pre-mRNA splicing, is crucial for shaping plant responses.
  • Alternative splicing (AS) diversifies the proteome, with 80% of plant genes exhibiting this phenomenon.

Purpose of the Study:

  • To review the mechanisms of pre-mRNA splicing and AS in plants, focusing on stress responses.
  • To explore the potential of AS in engineering stress-resilient crops for sustainable agriculture.

Main Methods:

  • Review of existing literature on plant splicing mechanisms.
  • Discussion of CRISPR-Cas technologies for dissecting AS regulation.
  • Analysis of AS's role in plant development and stress adaptation.

Main Results:

  • Alternative splicing is a key mechanism for plant adaptation to environmental stresses.
  • CRISPR-Cas technologies provide powerful tools to investigate AS.
  • AS plays a significant role in plant development and stress responses.

Conclusions:

  • Insights into AS mechanisms can be leveraged to engineer crops with improved stress resilience.
  • Harnessing AS offers a pathway toward sustainable agriculture in a changing climate.