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pH Regulation in Cells

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Related Experiment Video

Updated: May 27, 2025

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Published on: December 12, 2015

Lipid- and Multivariate-Based Analyses to Determine Cell Response to pH Variations and Buffer Composition.

Ricardo F S Pereira^1,2, Carla C C R de Carvalho^3,4

¹iBB-Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001, Lisbon, Portugal.

Marine Biotechnology (New York, N.Y.)

|February 15, 2025

Summary

Good's buffers offer a viable alternative to traditional phosphate buffers in marine bioprocesses. Specific buffers like MES and EPPS significantly enhanced prodigiosin production by optimizing pH control.

Keywords:

Biological buffer, Prodigiosin Marine medium Multivariate statistical analysis Phosphate buffer pH

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Area of Science:

Biotechnology
Microbial Physiology
Biochemical Engineering

Background:

pH control is critical in marine bioprocesses, but traditional buffers like phosphate can cause precipitation.
Aerobic fermentations in shake flasks often lack active pH control, relying solely on medium buffering capacity.

Purpose of the Study:

To evaluate Good's buffers as alternatives to phosphate buffer in marine bioprocesses.
To investigate the impact of different buffers and pH levels on prodigiosin production by Serratia rubidaea.
To understand cellular responses, specifically lipidomic changes, to varying buffer compositions.

Main Methods:

Serratia rubidaea fermentation using various Good's buffers (MES, EPPS, HEPES, TRIS) and phosphate buffer at different pH values.
Lipidomics analysis to assess cellular membrane composition.
Statistical multivariate analysis to interpret lipidomic data and correlate with production metrics.

Main Results:

Biomass productivity remained consistent across different buffers.
Prodigiosin production was significantly influenced by buffer type and pH, with MES at pH 5.5 yielding the highest yield (249.8 mg/L).
EPPS, HEPES, and TRIS buffers at pH 7.0 proved effective substitutes for phosphate buffer in marine media.
Cells demonstrated adaptability by altering membrane fatty acid composition in response to buffer species.

Conclusions:

Good's buffers are effective alternatives to phosphate buffers, enabling enhanced metabolite production.
Buffer selection and pH optimization are crucial factors for successful marine bioprocess development.
Cellular adaptation to buffer composition highlights the importance of considering buffer-microbe interactions.