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Updated: May 27, 2025
Test Samples for Optimizing STORM Super-Resolution Microscopy
Published on: September 6, 2013
Correlation-based nanometric localization using lattice SIM2 and dSTORM in PML nuclear bodies validated by
Corentin Rousset1, Rémi Neplaz1, Pelin Catal2
1Université Claude Bernard Lyon 1, Laboratoire Physiopathologie et Génétique du Neurone et du Muscle-Institut NeuroMyoGène, Team « Chromatin dynamics, nuclear domains, virus », PGNM-INMG, UCBL - CNRS UMR5261 - INSERM U1315, LAbEx Dev2CAN, Faculté de Médecine et de Pharmacie, F69008, Lyon, France.
Abstract:
We evaluated the Lattice di-SIM 3D structural illumination method with a focus on Promyelocytic Leukemia Nuclear Bodies (PML NBs). Lattice SIM2's performance was compared to its predecessor using biological samples and calibration beads. Optimized SIM2 parameters revealed PML NBs with a distinct ring-like morphology. Furthermore, we compared Lattice-SIM2 with dSTORM, finding a strong correlation between blinking events and the SIM2 mask. For 2D nanoscopic correlation, we used the long-lived imaging buffer Eternity, while the modified variant, Eternity-SIM, with a higher refractive index, provided improved 3D correlation for SIM2 imaging.
