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Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

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Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
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Atomic Emission Spectroscopy: Lab01:29

Atomic Emission Spectroscopy: Lab

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AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...
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Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

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Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
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Atomic Absorption Spectroscopy: Lab01:21

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For AAS measurements, samples must be introduced as clear solutions, often requiring extensive preliminary treatment to dissolve materials like soils, animal tissues, and minerals. Common methods for sample preparation include treatment with hot mineral acids, wet ashing, combustion in closed containers, high-temperature ashing, or fusion with reagents.
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Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

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Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
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Laser-ablation vs. bulk tissue ICP-MS for conifer tissue elemental analysis.

Jasmine M Williams1, Sean C Thomas2

  • 1Institute of Forestry and Conservation, University of Toronto, 33 Willcocks St, Toronto, M5S 3B3, Canada; Department of Civil, Geological, and Mining Engineering, École Polytechnique de Montreal, 2500 chemin de la Polytechnique, Montreal, H3T 1J4, Canada.

Chemosphere
|February 15, 2025
PubMed
Summary

Laser ablation inductively coupled plasma mass spectrometry (LA ICP-MS) offers accurate, non-destructive elemental analysis of intact plant tissues. This method is ideal for small samples and volatile elements, outperforming conventional techniques.

Keywords:
Elemental analysisJack pineLA ICP-MSLaser ablationPhytoaccumulationTrace elementsTree tissue

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Plant Biology

Background:

  • Conventional bulk sample analysis for trace elements involves sample preparation that risks contamination and loss.
  • Laser ablation inductively coupled plasma mass spectrometry (LA ICP-MS) offers direct elemental analysis of solid samples with minimal preparation.
  • Applying LA ICP-MS to plant tissues faces challenges in heterogeneity, calibration standards, and sample preparation protocols.

Purpose of the Study:

  • To evaluate the accuracy and applicability of LA ICP-MS for analyzing elemental composition in intact jack pine (Pinus banksiana L.) needles.
  • To compare LA ICP-MS analysis of intact needles with pulverized samples and conventional acid digestion-ICP-MS methods.
  • To assess the suitability of LA ICP-MS for analyzing various elements, including nutrients and volatile metals, in plant tissues.

Main Methods:

  • Analysis of jack pine needles using LA ICP-MS on both intact and pulverized samples.
  • Utilized NIST SRM 610 quartz standard for calibration and 43Ca as an internal standard.
  • Compared LA ICP-MS results with conventional aqua regia acid digestion followed by ICP-MS analysis.

Main Results:

  • LA ICP-MS analysis of intact pine needles accurately predicted elemental composition for most elements compared to acid digestion methods.
  • Pulverizing and pelletizing samples did not improve accuracy or reduce bias compared to intact samples.
  • LA ICP-MS showed close agreement for plant nutrients (P, Mg, Zn) but deviated for less-stable metals (Ce, La, Fe) and volatile elements (Pb).
  • Potassium (K) values varied due to preferential localization in mesophyll layers beyond laser ablation depth.

Conclusions:

  • LA ICP-MS is a viable tool for accurate, non-destructive elemental analysis of intact plant tissues.
  • The method is particularly advantageous for analyzing small sample sizes and volatile elements.
  • Intact tissue analysis by LA ICP-MS provides reliable data, especially for elements not amenable to conventional methods.