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Updated: May 27, 2025

Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry LA-ICP-MS
Published on: January 22, 2017
Laser-ablation vs. bulk tissue ICP-MS for conifer tissue elemental analysis
Jasmine M Williams1, Sean C Thomas2
1Institute of Forestry and Conservation, University of Toronto, 33 Willcocks St, Toronto, M5S 3B3, Canada; Department of Civil, Geological, and Mining Engineering, École Polytechnique de Montreal, 2500 chemin de la Polytechnique, Montreal, H3T 1J4, Canada.
Laser ablation inductively coupled plasma mass spectrometry (LA ICP-MS) offers accurate, non-destructive elemental analysis of intact plant tissues. This method is ideal for small samples and volatile elements, outperforming conventional techniques.
Area of Science:
- Environmental Science
- Analytical Chemistry
- Plant Biology
Background:
- Conventional bulk sample analysis for trace elements involves sample preparation that risks contamination and loss.
- Laser ablation inductively coupled plasma mass spectrometry (LA ICP-MS) offers direct elemental analysis of solid samples with minimal preparation.
- Applying LA ICP-MS to plant tissues faces challenges in heterogeneity, calibration standards, and sample preparation protocols.
Purpose of the Study:
- To evaluate the accuracy and applicability of LA ICP-MS for analyzing elemental composition in intact jack pine (Pinus banksiana L.) needles.
- To compare LA ICP-MS analysis of intact needles with pulverized samples and conventional acid digestion-ICP-MS methods.
- To assess the suitability of LA ICP-MS for analyzing various elements, including nutrients and volatile metals, in plant tissues.
Main Methods:
- Analysis of jack pine needles using LA ICP-MS on both intact and pulverized samples.
- Utilized NIST SRM 610 quartz standard for calibration and 43Ca as an internal standard.
- Compared LA ICP-MS results with conventional aqua regia acid digestion followed by ICP-MS analysis.
Main Results:
- LA ICP-MS analysis of intact pine needles accurately predicted elemental composition for most elements compared to acid digestion methods.
- Pulverizing and pelletizing samples did not improve accuracy or reduce bias compared to intact samples.
- LA ICP-MS showed close agreement for plant nutrients (P, Mg, Zn) but deviated for less-stable metals (Ce, La, Fe) and volatile elements (Pb).
- Potassium (K) values varied due to preferential localization in mesophyll layers beyond laser ablation depth.
Conclusions:
- LA ICP-MS is a viable tool for accurate, non-destructive elemental analysis of intact plant tissues.
- The method is particularly advantageous for analyzing small sample sizes and volatile elements.
- Intact tissue analysis by LA ICP-MS provides reliable data, especially for elements not amenable to conventional methods.
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