Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Omni-domain energy-efficient decision-making for large-scale heterogeneous platoons with dual-level graph reinforcement learning.

Neural networks : the official journal of the International Neural Network Society·2026
Same author

A testing method for aeroengine casings under high-temperature aerodynamic fatigue loads.

The Review of scientific instruments·2026
Same author

Direct and Continuous Monitoring of Multicomponent Antibiotic Gentamicin in Blood at Single-Molecule Resolution.

ACS nano·2024
Same author

Direct single-molecule detection of CoA-SH and ATP by the membrane proteins TMEM120A and TMEM120B.

Nanoscale·2024
Same author

Real-time detection of 20 amino acids and discrimination of pathologically relevant peptides with functionalized nanopore.

Nature methods·2024
Same author

Can inactivation mutation in the thyroid stimulating hormone receptor gene and hyperthyroidism coexist?: A case report.

Medicine·2024

Related Experiment Video

Updated: May 27, 2025

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.7K

Pore lipid modifications modulate MscS nanopore for enhanced single-molecule sensing.

Changjian Zhao1, Xingyu Mou1, Qianqian Zhang2

  • 1Center of Infectious Diseases, Division of Infectious Diseases in State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, Sichuan, 610041, China.

Biosensors & Bioelectronics
|February 16, 2025
PubMed
Summary

Researchers modified the MscS channel using detergents to alter its properties. This convenient method enables selective single-molecule detection without complex protein engineering.

Keywords:
PaMscS nanoporePore lipid modificationSingle molecule sensing

More Related Videos

High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

5.7K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

13.4K

Related Experiment Videos

Last Updated: May 27, 2025

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
11:55

Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution

Published on: August 16, 2016

11.7K
High Resolution Physical Characterization of Single Metallic Nanoparticles
09:56

High Resolution Physical Characterization of Single Metallic Nanoparticles

Published on: June 28, 2019

5.7K
Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores
09:43

Fine-tuning the Size and Minimizing the Noise of Solid-state Nanopores

Published on: October 31, 2013

13.4K

Area of Science:

  • Biophysics
  • Nanotechnology
  • Molecular Biology

Background:

  • Transmembrane channel modification is crucial for advanced functionality.
  • Existing methods involve complex chemical reactions or protein engineering.
  • The MscS channel's pore lipids offer a site for functional tuning.

Purpose of the Study:

  • To develop a simplified method for modifying MscS channel properties.
  • To investigate the impact of detergent-induced pore lipid replacement on MscS function.
  • To enable selective single-molecule detection using engineered MscS channels.

Main Methods:

  • Utilized lipid-like molecules (detergents) to replace native pore lipids in MscS.
  • Determined the structure of modified MscS using Cryo-Electron Microscopy (Cryo-EM).
  • Performed single-channel recordings and molecular dynamics simulations to analyze functional changes.

Main Results:

  • Confirmed stable binding of detergents (DDM) at the MscS hydrophobic pathway entrance.
  • Observed altered channel conductance and gating behavior due to detergent substitution.
  • Demonstrated that detergent modifications adjust TM1-TM2 helix tilt, influencing channel state.

Conclusions:

  • Detergent modification provides a facile and efficient route for MscS nanopore engineering.
  • Tailored MscS channels exhibit selective single-molecule detection capabilities.
  • This approach bypasses the need for intricate chemical synthesis or protein recombination.