Development and functionality analysis of lipoplex-loaded polysaccharide-based surface coatings for local nucleic...

Area of Science:

• Biomaterials Science
• Drug Delivery
• Nanotechnology

Background:

• Therapeutic nucleic acids require effective delivery strategies for clinical success.
• Local delivery of nucleic acids offers spatially controlled therapeutic action but is underexplored.
• Existing local nucleic acid delivery systems are limited in description and application.

Purpose of the Study:

• To design and characterize novel polysaccharide-based surface coatings for local nucleic acid delivery.
• To investigate the impact of manufacturing parameters on coating properties and nucleic acid carrier integration.
• To evaluate the efficiency and mechanism of nucleic acid transfer and release from the developed system.

Main Methods:

• Automatized Layer-by-Layer deposition of hyaluronic acid and chitosan to create polyelectrolyte multilayer systems.
• Embedding of lipid nanoparticles (lipoplexes) as nucleic acid carriers within the multilayer films.
• Systematic investigation of film properties (thickness, topology, lipoplex distribution) and in vitro transfection efficiency.

Main Results:

• Polysaccharide coatings prepared in acetate buffer showed optimal lipoplex embedding and uniform distribution.
• The developed system demonstrated efficient, contact-triggered nucleic acid transfer into cells.
• Enzymatic degradation by hyaluronidase facilitated cargo release and successful in vitro transfection.

Conclusions:

• Polysaccharide-based multilayer coatings are a promising platform for local nucleic acid delivery.
• Acetate buffer optimization is crucial for achieving effective lipoplex integration and uniform distribution.
• The system enables controlled release and efficient cellular uptake of nucleic acids for therapeutic applications.